Enzymatic (2R,4R)‐Pentanediol Synthesis – “Putting a Bottle on the Table”

Author:

Sehl Torsten1,Seibt Lisa1ORCID,Kappauf Katrin1,Ergezinger Pia1,Spöring Jan-Dirk1ORCID,Mielke Kristina2,Doeker Moritz2,Verma Neha3,Bocola Marco3,Daußmann Thomas3,Chen Haibin4ORCID,Shi Shumin4,Jupke Andreas2ORCID,Rother Dörte15ORCID

Affiliation:

1. Forschungszentrum Jülich GmbH Institute of Bio- and Geosciences, IBG-1: Biotechnology Leo-Brandt-Straße 1 52425 Jülich Germany

2. RWTH Aachen University AVT.FVT – Fluid Process Engineering Forckenbeckstraße 51 52074 Aachen Germany

3. Enzymaster Deutschland GmbH Neusser Straße 39 40219 Düsseldorf Germany

4. Enzymaster (Ningbo) Bioengineering Co Ltd. 333 North Century Avenue 315042 Ningbo China

5. RWTH Aachen University Aachen Biology and Biotechnology (ABBt) Worringer Weg 1 52074 Aachen Germany

Abstract

Abstract(2R,4R)‐Pentanediol is an interesting precursor for the synthesis of chiral ligands. A ketoreductase (KRED) was employed for the asymmetric reduction of acetylacetone to this diol. Biocatalysis often suffers from low concentrations of hydrophobic substrates and low stability of the enzyme in unconventional media. Here, we present an engineered KRED variant applicable in a neat substrate system, including upscaling to the multi‐liter scale and downstream processing (DSP). Our engineered KRED applied in a neat substrate system is a powerful technique for the synthesis of chiral diols yielding product concentrations of 208 g L−1.

Publisher

Wiley

Subject

Industrial and Manufacturing Engineering,General Chemical Engineering,General Chemistry

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