Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: Comparison with TGF-β1-Reference-Cited by-同舟云学术

Bone morphogenetic protein-2b stimulation of growth and osteogenic phenotypes in rat osteoblast-like cells: Comparison with TGF-β1

Published:1991-12-01 Issue:12 Volume:6 Page:1387-1393
ISSN:0884-0431
Container-title:Journal of Bone and Mineral Research
language:en
Short-container-title:

Author:

Chen Theresa L.¹,Bates Rebecca L.¹,Dudley Andrew¹,Hammonds Glenn R.¹,Amento Edward P.¹

Affiliation:

1. Inflammation, Bone and Connective Tissue Research, Department of Developmental Biology, Genentech, Inc., South San Francisco, California

Abstract

Abstract The biologic effects of recombinant human bone morphogenetic protein-2b (BMP-2b = BMP-4) were studied and compared with transforming growth factor-β1 (TGF-β1) in fetal rat osteoblast-like (ROB) cells. Similar to the effects of TGF-β1, BMP-2b stimulated DNA and collagen synthesis as well as protein accumulation. Unlike TGF-β1, which inhibited alkaline phosphatase activity, BMP-2b enhanced enzyme activity eight-to ninefold over the control level. The present study demonstrates direct actions of BMP-2b on bone-associated cells to stimulate osteogenic phenotypes in vitro and provides a cellular mechanism for the induction of bone formation by BMP-2b in vivo.

Publisher

Oxford University Press (OUP)

Link

https://academic.oup.com/jbmr/article-pdf/6/12/1387/56432205/5650061216.pdf

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