UGT201H1 overexpression confers cyflumetofen resistance in Tetranychus cinnabarinus (Boisduval)

Author:

Wen Xiang123,Chen Yini123,Chen Qingying123,Tang Xuejing123,Feng Kaiyang123,He Lin123ORCID

Affiliation:

1. Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection Southwest University Chongqing China

2. Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River Ministry of Education Chongqing China

3. National Citrus Engineering Research Center Southwest University Chongqing China

Abstract

AbstractBACKGROUNDTetranychus cinnabarinus is one of the most common polyphagous arthropod herbivores, and is primarily controlled by the application of acaricides. The heavy use of acaricides has led to high levels of resistance to acaricides such as cyflumetofen, which poses a threat to global resistance management programs. Cyflumetofen resistance is caused by an increase in metabolic detoxification; however, the role of uridine diphosphate (UDP)‐glycosyltransferase (UGT) genes in cyflumetofen resistance remains to be determined.RESULTSSynergist 5‐nitrouracil (5‐Nul) significantly enhanced cyflumetofen toxicity in T. cinnabarinus, which indicated that UGTs are involved in the development of cyflumetofen resistance. Transcriptomic analysis and quantitative (q)PCR assays demonstrated that the UGT genes, especially UGT201H1, were highly expressed in the YN‐CyR strain, compared to those of the YN‐S strain. The RNA interference (RNAi)‐mediated knockdown of UGT201H1 expression diminished the levels of cyflumetofen resistance in YN‐CyR mites. The findings additionally revealed that the recombinant UGT201H1 protein plays a role in metabolizing cyflumetofen. Our results also suggested that the aromatic hydrocarbon receptor (AhR) probably regulates the overexpression of the UGT201H1 detoxification gene.CONCLUSIONUGT201H1 is involved in cyflumetofen resistance, and AhR may regulates the overexpression of UGT201H1. These findings provide deeper insights into the molecular mechanisms underlying UGT‐mediated metabolic resistance to chemical insecticides. © 2024 Society of Chemical Industry.

Funder

National Natural Science Foundation of China

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3