Affiliation:
1. Department of Chemical and Biomolecular Engineering University of Nebraska-Lincoln Lincoln N-68588 USA
2. The Nebraska Center for Integrated Biomolecular Communication (NCIBC) University of Nebraska-Lincoln Lincoln NE-68588 USA
3. Department of Chemistry University of Nebraska-Lincoln Lincoln NE-68588 USA
Abstract
AbstractProtein posttranslational modifications (PTMs) play critical roles in regulating cellular activities. Here we provide a survey of genetic code expansion (GCE) methods that were applied in the co‐translational installation and studies of PTMs through noncanonical amino acid (ncAA) mutagenesis. We begin by reviewing types of PTM that have been installed by GCE with a focus on modifications of tyrosine, serine, threonine, lysine, and arginine residues. We also discuss examples of applying these methods in biological studies. Finally, we end the piece with a short discussion on the challenges and the opportunities of the field.
Funder
Nebraska Center for Integrated Biomolecular Communication
Subject
Organic Chemistry,Molecular Biology,Molecular Medicine,Biochemistry