Affiliation:
1. College of Chemistry and Materials Science Shanghai Normal University Shanghai 200233 China
2. Institute of Molecular Medicine Department of Laboratory Medicine Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine Renji Hospital School of Medicine Shanghai Jiao Tong University Shanghai 200127 China
3. School of Life Sciences Shanghai University Shanghai 200444 China
Abstract
AbstractBiomarkers are crucial physiological and pathological indicators in the host. Over the years, numerous detection methods have been developed for biomarkers, given their significant potential in various biological and biomedical applications. Among these, the detection system based on functionalized DNA origami has emerged as a promising approach due to its precise control over sensing modules, enabling sensitive, specific, and programmable biomarker detection. We summarize the advancements in biomarker detection using functionalized DNA origami, focusing on strategies for DNA origami functionalization, mechanisms of biomarker recognition, and applications in disease diagnosis and monitoring. These applications are organized into sections based on the type of biomarkers – nucleic acids, proteins, small molecules, and ions – and concludes with a discussion on the advantages and challenges associated with using functionalized DNA origami systems for biomarker detection.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Innovative Research Team of High-level Local University in Shanghai