Locational and functional characterization of PI4KB in the mouse embryo

Author:

Zhao Haoyu1,Kong Fengyun2,Yu Weikai1,Zhao Huijie1ORCID,Zhang Jingjing3,Zhou Jun1ORCID,Meng Xiaoqian1ORCID

Affiliation:

1. Shandong Provincial Key Laboratory of Animal Resistance Biology Institute of Biomedical Sciences, College of Life Sciences, Centre for Cell Structure and Function, Shandong Normal University Jinan China

2. Reproductive Medical Center, The Second Hospital Affiliated to Shandong University of Traditional Chinese Medicine Jinan China

3. Affiliated Hospital of Guangdong Medical University & Key Laboratory of Zebrafish Model for Development and Disease of Guangdong Medical University Zhanjiang China

Abstract

AbstractPhosphatidylinositol 4‐kinase beta (PI4KB) is a member of the PI4K family, which is mainly enriched and functions in the Golgi apparatus. The kinase domain of PI4KB catalyzes the phosphorylation of phosphatidylinositol to form phosphatidylinositol 4‐phosphate, a process that regulates various sub‐cellular events, such as non‐vesicular cholesterol and ceramide transport, protein glycosylation, and vesicle transport, as well as cytoplasmic division. In this study, a strain of PI4KB knockout mouse, immunofluorescence, reverse transcription polymerase chain reaction and microinjection were used to characterize the cytological location and biological function of PI4KB in the mouse embryos. we found that knocking down Pi4kb in mouse embryos resulted in embryonic lethality at around embryonic day (E) 7.5. Additionally, we observed dramatic fluctuations in PI4KB expression during the development of preimplantation embryos, with high expression in the 4‐cell and morula stages. PI4KB colocalized with the Golgi marker protein TGN46 in the perinuclear and cytoplasmic regions in early blastomeres. Postimplantation, PI4KB was highly expressed in the epiblast of E7.5 embryos. Treatment of embryos with PI4KB inhibitors was found to inhibit the development of the morula into a blastocyst and the normal progression of cytoplasmic division during the formation of a 4‐cell embryo. These findings suggest that PI4KB plays an important role in mouse embryogenesis by regulating various intracellular vital functions of embryonic cells.

Publisher

Wiley

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1. Phosphatidyl Inositol 4-Kinases;Encyclopedia;2024-06-29

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