Affiliation:
1. School of Biomedical Engineering Shenzhen Campus of Sun Yat‐sen University Shenzhen 518107 China
2. Key Laboratory of Sensing Technology and Biomedical Instruments of Guangdong Province School of Biomedical Engineering Sun Yat‐sen University Guangzhou 510275 China
3. Department of Emergency Nanfang Hospital Southern Medical University Guangzhou 510515 China
Abstract
AbstractLateral‐flow assay (LFA) is one of the most commonly used detection technologies, in which the chromatographic membranes are currently used as the lateral‐flow membrane (e.g., nitrocellulose membrane, NC Mem). However, several disadvantages of existing chromatographic membranes limit the performance of LFA, including relatively low flow velocity of sample solution and relatively more residuals of sample on membrane, which increase detection time and detection noise. Herein, a surface structure membrane (SS Mem) is proposed, which enables fast self‐transport of water with a convection manner and realizes low residuals of sample on membrane surface after the flow. On SS Mem, the flow velocity of water is 7.1‐fold higher, and the residuals of sample are decreased by 60–67%, comparing those in NC Mem. SS Mem is used as lateral‐flow membrane to prepare lateral‐flow strips of nanogold LFA and fluorescence LFA for rapid detection of SARS CoV‐2 nucleocapsid protein. These LFAs require 210 s per detection, with limits of detection of 3.98 pg mL−1 and 53.3 fg mL−1, sensitivity of 96.5%, and specificity of 90%. The results suggest that SS Mem enables ultrafast, highly sensitive lateral‐flow immunoassays and shows great potential as a new type of lateral‐flow membrane to broaden the application of LFA.
Funder
National Natural Science Foundation of China
Guangdong Provincial Key Laboratory of Sensor Technology and Biomedical Instrument
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry