Affiliation:
1. College of Materials Science and Engineering Qingdao University of Science and Technology 53 Zhengzhou Road Qingdao Shandong 266042 China
2. College of Chemical Engineering Qingdao University of Science and Technology 53 Zhengzhou Road Qingdao Shandong 266042 China
3. Central Laboratory Huashan Hospital Fudan University 12 Middle Urumqi Road Shanghai 200040 China
Abstract
AbstractKeratitis, an inflammation of the cornea caused by bacterial or fungal infections, is one of the leading causes of severe visual disability and blindness. Keratitis treatment requires both the prevention of infection and the reduction of inflammation. However, owing to their limited therapeutic functions, in addition to the ocular barrier, existing conventional medications are characterized by poor efficacy and low bioavailability, requiring high dosages or frequent topical treatment, which represents a burden on patients and increases the risk of side effects. In this study, manganese oxide nanocluster‐decorated graphdiyne nanosheets (MnOx/GDY) are developed as multienzyme‐like nanozymes for the treatment of infectious keratitis and loaded into hyaluronic acid and polymethyl methacrylate‐based ocular microneedles (MGMN). MGMN not only exhibits antimicrobial and anti‐inflammatory effects owing to its multienzyme‐like activities, including oxidase, peroxidase, catalase, and superoxide dismutase mimics but also crosses the ocular barrier and shows increased bioavailability via the microneedle system. Moreover, MGMN is demonstrated to eliminate pathogens, prevent biofilm formation, reduce inflammation, alleviate ocular hypoxia, and promote the repair of corneal epithelial damage in in vitro, ex vivo, and in vivo experiments, thus providing a better therapeutic effect than commercial ophthalmic voriconazole, with no obvious microbial resistance or cytotoxicity.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Shandong Province
Subject
Biomaterials,Biotechnology,General Materials Science,General Chemistry