Affiliation:
1. Novartis Institutes for BioMedical Research Cambridge Massachusetts USA
2. Amgen Research Thousand Oaks California USA
3. Novartis Institutes for BioMedical Research Basel Switzerland
Abstract
AbstractIn this study, crown ether‐derived column Crownpak® CR‐I (+) was evaluated under SFC conditions using 12 primary amines, and the chromatographic results were compared against eight immobilized polysaccharide‐based columns. Crownpak® CR‐I (+) achieved a significantly higher success rate. It was found that the addition of 5% water to the modifier dramatically improved the peak shape for chiral separation of primary amines on Crownpak® CR‐I (+). The first reported preparative SFC separations on Crownpak® CR‐I (+) are shown, offering a new approach for the preparative resolution of primary amines. The case studies demonstrate that Crownpak® CR‐I (+) is a very useful column in the chiral separation of challenging compounds that contain a primary amine group in the pharmaceutical industry.
Subject
Organic Chemistry,Spectroscopy,Drug Discovery,Pharmacology,Catalysis,Analytical Chemistry