Affiliation:
1. Department of Experimental Pharmacology and Toxicology, School of Pharmacy Jilin University Changchun China
2. Department of Ophthalmology The First Hospital of Jilin University Changchun China
3. Department of Hepatobiliary Surgery Songyuan Central Hospital Songyuan China
Abstract
AbstractObjectivePirarubicin (THP) is a widely used antitumor drug in clinical practice, but its cardiotoxicity limits its use. There is an urgent need to find drugs to alleviate the cardiotoxicity of THP. This study aimed to investigate the effect and mechanism of miR‐494‐3p on THP‐induced cardiomyocytes.MethodsTHP induced immortalized mouse cardiomyocytes HL‐1, silenced or overexpressed miR‐494‐3p. The effects of miR‐494‐3p on HL‐1 contained in THP were investigated by CCK8, flow cytometry, ROS detection, JC‐1 mitochondrial membrane potential detection, TUNEL cell apoptosis detection, RT‐qPCR, and Western blot.ResultsmiR‐494‐3p could reduce cell viability, increase oxidative damage, and promote cell apoptosis; at the same time, it inhibited the expression of MDM4, promoted the activation of p53, and promoted the expression of apoptosis‐related proteins. MiR‐494‐3p inhibitors have the opposite effect.ConclusionmiR‐494‐3p can aggravate THP damage to HL‐1, which may be achieved by downregulating MDM4 and promoting p53. miR‐494‐3p is one of the important miRNAs in THP‐induced cardiotoxicity, which provides theoretical support for its possible use as a therapeutic target for THP‐induced cardiovascular disease.
Funder
National Natural Science Foundation of China
Subject
Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献