<scp>TRBC1</scp> in flow cytometry: Assay development, validation, and reporting considerations-Reference-Cited by-同舟云学术

TRBC1 in flow cytometry: Assay development, validation, and reporting considerations

Published:2024-05 Issue:3 Volume:106 Page:192-202
ISSN:1552-4949
Container-title:Cytometry Part B: Clinical Cytometry
language:en
Short-container-title:Cytometry Part B Clinical

Author:

Devitt Katherine A.¹²^ORCID,Kern Wolfgang³,Li Weijie⁴^ORCID,Wang Xuehai⁵,Wong Allyson J.⁶,Furtado Felipe M.⁷⁸,Oak Jean S.⁹,Illingworth Andrea¹⁰

Affiliation:

1. Department of Pathology and Laboratory Medicine University of Vermont Medical Center Burlington Vermont USA

2. Larner College of Medicine at the University of Vermont Burlington Vermont USA

3. Department of Flow Cytometry MLL Munich Leukemia Laboratory Munich Germany

4. Department of Pathology and Laboratory Medicine, Children's Mercy Hospital University of Missouri‐Kansas City School of Medicine Kansas City Missouri USA

5. Division of Hematopathology Vancouver General Hospital Vancouver British Columbia Canada

6. Pathology and Laboratory Medicine Lahey Hospital and Medical Center Burlington Massachusetts USA

7. Hematology Department Sabin Diagnostico e Saude Brasília Brazil

8. Oncohematology Department Hospital da Criança de Brasília Brasília Brazil

9. Department of Pathology Stanford University Stanford California USA

10. Department of Flow Cytometry Dahl‐Chase Diagnostic Services/Versant Diagnostics Bangor Maine USA

Abstract

AbstractThe assessment of T‐cell clonality by flow cytometry has long been suboptimal, relying on aberrant marker expression and/or intensity. The introduction of TRBC1 shows much promise for improving the diagnosis of T‐cell neoplasms in the clinical flow laboratory. Most laboratories considering this marker already have existing panels designed for T‐cell workups and will be determining how best to incorporate TRBC1. We present this comprehensive summary of TRBC1 and supplemental case examples to familiarize the flow cytometry community with its potential for routine application, provide examples of how to incorporate it into T‐cell panels, and signal caution in interpreting the results in certain diagnostic scenarios where appropriate.

Publisher

Wiley

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/cyto.b.22175

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