MicroRNA‐145‐5p suppresses cell proliferation, migration, and invasion in upper tract urothelial carcinoma by targeting 5‐aminoimidazole‐4‐carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase

Abstract

AbstractUpper tract urothelial carcinoma (UTUC), including renal, pelvic, and ureteral carcinoma, has a high incidence rate in Taiwan, which is different from that in Western countries. Therefore, it is imperative to elucidate the mechanisms underlying UTUC growth and metastasis. To explore the function of miR‐145‐5p in UTUC, we transfected the BFTC909 cell line with miR‐145‐5p mimics and analyzed the differences in protein levels by performing two‐dimensional polyacrylamide gel electrophoresis. Real‐time polymerase chain reaction and Western blot analysis were used to analyze 5‐aminoimidazole‐4‐carboxamide ribonucleotide formyltransferase/inositol monophosphate cyclohydrolase (ATIC) messenger RNA and protein levels. A dual‐luciferase assay was performed to identify the target of miR‐145‐5p in ATIC. The effects of miR‐145‐5p and ATIC expression by cell transfection on cell proliferation, migration, and invasion were also assessed. miR‐145‐5p downregulated ATIC protein expression. High ATIC expression is associated with tumor stage, metastasis, recurrence, and a poor prognosis in patients with UTUC. Cell function assays revealed that ATIC knockdown inhibited the proliferation, migration, and invasive abilities of UTUC cells. In contrast, miR‐145‐5p affected the proliferation, migration, and invasive abilities of UTUC cells by directly targeting the 3′‐untranslated regions of ATIC. Furthermore, we used RNA sequencing and Ingenuity Pathway Analysis to identify possible downstream genes regulated by ATIC and found that miR‐145‐5p regulated the protein levels of fibronectin 1, Slug, cyclin A2, cyclin B1, P57, and interferon‐induced transmembrane 1 via ATIC. ATIC may be a valuable predictor of prognosis and a potential therapeutic target for UTUC.