Sinensetin ameliorates high glucose‐induced diabetic nephropathy via enhancing autophagy in vitro and in vivo

Author:

Kong Zili12,Lv Wenshan1,Wang Yunyang1,Huang Yajing1,Che Kui12,Nan Huiqi1,Xin Yu1,Wang Jiaxuan1,Chen Jintao1,Wang Yangang1,Chi Jingwei12ORCID

Affiliation:

1. Department of Endocrinology and Metabolism Affiliated Hospital of Qingdao University Qingdao China

2. Department of Endocrinology and Metabolism, Qingdao Key Laboratory of Thyroid Diseases, Medical Research Center Affiliated Hospital of Qingdao University Qingdao China

Abstract

AbstractDiabetic nephropathy (DN) affects around 40% of people with diabetes, the final outcome of which is end‐stage renal disease. The deficiency of autophagy and excessive oxidative stress have been found to participate in the pathogenesis of DN. Sinensetin (SIN) has been proven to have strong antioxidant capability. However, the effect of SIN on DN has not been studied. We examined the effect of SIN on cell viability and autophagy in the podocyte cell line, MPC5 cells, treated with high glucose (HG). For in vivo studies, DN mice models were established by intraperitoneal injected with streptozotocin (40 mg/kg) for 5 consecutive days and fed with a 60% high‐fat diet, and SIN was given (10, 20, and 40 mg/kg) for 8 weeks via intraperitoneal injection. The results showed that SIN could protect MPC5 cells against HG‐induced damage and significantly improve the renal function of DN mice. Moreover, SIN remarkably restored the autophagy activity of MPC5 cells which was inhibited under HG conditions. Consistent with this, SIN efficiently improved autophagy in the kidney tissue of DN mice. In brief, our findings demonstrated the protective effect of SIN on DN via restoring the autophagic function, which might provide a basis for drug development.

Funder

National Natural Science Foundation of China

Natural Science Foundation of Shandong Province

Publisher

Wiley

Subject

Health, Toxicology and Mutagenesis,Toxicology,Molecular Biology,Molecular Medicine,Biochemistry,General Medicine

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