Affiliation:
1. Department of Chemistry Institute of Technical Education and Research (FET) Siksha ‘O' Anusandhan, deemed to be University Khandagiri Square Bhubaneswar 751030 Odisha India
2. Laboratory Hematology Division Department of Pathology IMS & Sum Hospital Siksha ‘O' Anusandhan, deemed to be University Kalinga Nagar Bhubaneswar 751003 Odisha India
3. Laboratory of Biomaterials and Regenerative Medicine for Advanced Therapies ICMR-Regional Medical Research Center Bhubaneswar, 751 023 Odisha India
Abstract
AbstractThis study aims to gain insight into the effect of the synthesized poly‐ionic liquids (PILs) (1‐vinyl‐3‐alkyl imidazolium bromide) (alkyl=hexyl (A), octyl (B), and decyl (C)) on various cellular components of blood by flow cytometry. PILs may have some adverse effect on blood depending on the nature and the dose administered. So, it is critical to understand the potential influences of foreign polymeric entities on blood before they are used clinically. The complete blood counts (CBCs) are done using an automated analyzer based on the flow cytometry principle. The leukocyte viability with different PIL samples is performed by flow cytometry using 7‐AAD. The auto‐hemolysis is performed by incubating the PILs with defibrinated blood. The CBC is mostly the same when incubated with various PILs. However, RBCs show an increase in mean corpuscular volume (MCV), indicating cellular swelling. The viability with phosphate saline buffer (PBS), dimethyl sulphoxide (DMSO), and PIL (A, B, and C) is 99.94 %, 99.76 %, 99.71 %, 98.87 %, and 99.42 %, respectively. The effect on leukocyte size & internal structures are noted. The auto‐hemolysis tests of RBC by PILs are very similar to control auto‐hemolysis.