Affiliation:
1. Department of Chemistry, College of Science University of Basrah Basrah 61001 Iraq
2. Present address: Am Tannenhof 8 78464 Konstanz Germany
3. General Directorate of Muthana Education Muthana 66001 Iraq
4. Department of Chemistry University of Al-Qadisiya Al-Qadisiya 58001 Iraq
5. Department of Chemistry College of Science University of Kufa Kufa 54003 Iraq
6. Department of Chemistry College of Education for Pure Science University o Basrah Basrah 61001 Iraq
7. Department of Physiology, Pharmacology and Chemistry College of Veterinary University of Basrah Basrah 61001 Iraq
Abstract
AbstractHerein, a novel series of 1,5‐disubstituted‐1,2,3‐triazolines containing 1‐(4‐chlorobenzhydryl) piperazine moiety (8–18) were synthesised and evaluated for their anticancer activity across eight human tumor cell lines. Remarkably, compound 11 substituted with 3‐acetylphenyl group was the most potent anticancer agent against three selected human cancer cell lines (HL‐60, Z138, and DND‐41) with IC50 values of 16.80, 18.50, and 19.20 μM, respectively. In contrast, analogue 10 demonstrated activity against HL‐60, Z138, and DND‐41 cell lines, with IC50 values of 19.90, 18.00, and 18.50 μM, respectively. Moreover, derivative 13 substituted with 4‐bromophenyl moiety displayed activity with IC50=19.90 μM against the DND‐41 cell line. However, all analogues showed IC50 values ranging from 22.95 to 58.45 μM when tested against other investigated cancer cell lines. These findings suggest that derivative 11 holds promise as a potential candidate for synthesizing novel anticancer agents. Furthermore, compounds 8–18 were screened for their antioxidant activity. Molecular docking studies of compound 11 on crystal structures of two proteins, CDK2/cyclin A2 (PDB: 7B7S) and kinase Akt1 PKB alpha (PDB: 4GV1) have been studied.