Affiliation:
1. Department of Orthopaedics The First Affiliated Hospital of Soochow University Orthopaedic Institute of Soochow University Medical College of Soochow University 899 Pinghai Road Suzhou Jiangsu 215031 P. R. China
2. Department of Orthopaedics Shanghai Key Laboratory for Prevention and Treatment of Bone and Joint Diseases Shanghai Institute of Traumatology and Orthopaedics Ruijin Hospital Shanghai Jiao Tong University School of Medicine 197 Ruijin 2nd Road Shanghai 200025 P. R. China
3. Department of Orthopaedics The Second Affiliated Hospital of Soochow University 1055 Sanxiang Road Suzhou Jiangsu 215004 P. R. China
4. CAS Key Laboratory of Nano‐Bio Interface Suzhou Institute of Nano‐Tech and Nano‐Bionics Chinese Academy of Sciences 398 Ruoshui Road Suzhou Jiangsu 215123 P. R. China
5. Department of Orthopaedics Wuxi Ninth People's Hospital affiliated to Soochow University 999 Liangxi Road Wuxi Jiangsu 214026 P. R. China
Abstract
AbstractLentiviral‐vector‐based therapies, widely used for treating various diseases, face limitations because of release burst, rapid clearance, and immune activation. Herein, a lentiviral vector delivery platform is proposed utilizing a virus‐engineered microsol electrospun scaffold. Identifying a remarkable upregulation of polypyrimidine tract binding protein1 (PTB) in spinal cord injury (SCI) rats, a scaffold is constructed comprising a hyaluronic acid (HA) core encapsulating brain‐derived neurotrophic factor (BDNF) and a polydopamine (PDA)‐modified linear poly‐l‐lactic acid (PLLA) shell, with shPTB lentiviral vectors (LV‐shPTB) grafted via PDA. In vitro, the LV‐shPTB achieves an infection efficiency of 70%, and the oriented scaffolds significantly reduce the expression of inflammatory factors, induce the reprogramming of fibroblasts into neurons, and sustain the release of BDNF for over 2 weeks. In vivo, the scaffolds provide physical support and neural guidance, as well as released BDNF and LV‐shPTB. LV‐shPTB delivery leads to the reprogramming of fibroblasts into neurons, and the sustained BDNF delivery maintains the neurons’ proliferation and growth, which further promotes the recovery of neurological function in SCI rats. These results demonstrate the potential application of virus‐engineered delivery platforms in SCI treatment and other medical fields.
Funder
National Key Research and Development Program of China
National Natural Science Foundation of China
Priority Academic Program Development of Jiangsu Higher Education Institutions
Subject
Electrochemistry,Condensed Matter Physics,Biomaterials,Electronic, Optical and Magnetic Materials
Cited by
1 articles.
订阅此论文施引文献
订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献