Tracking the Endosomal Escape of Nanoparticles in Live Cells Using a Triplex‐Forming Oligonucleotide

Author:

Bhangu Sukhvir Kaur12ORCID,Mummolo Liviana2ORCID,Fernandes Soraia2ORCID,Amodio Alessia2ORCID,Radziwon Agata2ORCID,Dyett Brendan1ORCID,Savioli Marco23ORCID,Mantri Nitin1ORCID,Cortez‐Jugo Christina2ORCID,Caruso Frank2ORCID,Cavalieri Francesca13ORCID

Affiliation:

1. School of Science RMIT University Melbourne Victoria 3000 Australia

2. Department of Chemical Engineering The University of Melbourne Parkville Victoria 3010 Australia

3. Dipartimento di Scienze e Tecnologie Chimiche Università degli Studi di Roma “Tor Vergata” Via della Ricerca Scientifica 1 Rome 00133 Italy

Abstract

AbstractNanoparticle‐mediated intracellular delivery of oligonucleotides is a complex phenomenon that depends on the architecture and the intracellular trafficking of the engineered nanoparticles. Unravelling the molecular arrangements of oligonucleotides within the nanoparticles as well as their intracellular behavior are essential for designing effective nucleic acid delivery systems. Herein, a simple and general strategy for probing the endosomal escape of nanoparticles carrying oligonucleotides in live cells is reported. A triplex‐forming oligonucleotide probe is designed to target the transcription factor, kappa‐light‐chain‐enhancer of activated B cells (NF‐κB), in the cytosol of cells and to transduce the binding into a fluorescent Förster resonance energy transfer (FRET) signal. The combined use of the triplex‐forming oligonucleotide probe and super‐resolution microscopy enables the elucidation of the morphology, intracellular localization, and endosomal escape of the oligonucleotide‐loaded nanoparticles on a molecular level and with nanoscale resolution. The co‐delivery of the FRET probe and mRNA in cells via lipid‐ and polymer‐ based nanoparticles allow simultaneous correlation of the endosomal escape properties of nanoparticles and gene expression efficiency.

Funder

Australian Research Council

H2020 Marie Skłodowska-Curie Actions

National Health and Medical Research Council

Publisher

Wiley

Subject

Electrochemistry,Condensed Matter Physics,Biomaterials,Electronic, Optical and Magnetic Materials

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3