Affiliation:
1. Department of Neurology Mental and Neurological Disease Research Center the Third Affiliated Hospital of Sun Yat‐sen University Guangzhou 510630 China
2. Center of Clinical Immunology Mental and Neurological Disease Research Center the Third Affiliated Hospital of Sun Yat‐sen University Guangzhou 510630 China
3. Guangdong Provincial Key Laboratory of Brain Function and Disease Zhongshan School of Medicine Sun Yat‐sen University Guangzhou 510630 China
4. Surgical Intensive Care Unit The Third Affiliated Hospital of Sun Yat‐sen University Guangzhou 510630 China
Abstract
AbstractPneumonia is one of the leading causes of death in patients with acute ischemic stroke (AIS). Antibiotics fail to improve prognosis of patients with post‐stroke pneumonia, albeit suppressing infection, due to adverse impacts on the immune system. The current study reports that bone marrow mesenchymal stem cells (BM‐MSC) downregulate bacterial load in the lungs of stroke mice models. RNA‐sequencing of the lung from BM‐MSC‐treated stroke models indicates that BM‐MSC modulates pulmonary macrophage activities after cerebral ischemia. Mechanistically, BM‐MSC promotes the bacterial phagocytosis of pulmonary macrophages through releasing migrasomes, which are migration‐dependent extracellular vesicles. With liquid chromatography‐tandem mass spectrometry (LC‐MS/MS), the result shows that BM‐MSC are found to load the antibacterial peptide dermcidin (DCD) in migrasomes upon bacterial stimulation. Besides the antibiotic effect, DCD enhances LC3‐associated phagocytosis (LAP) of macrophages, facilitating their bacterial clearance. The data demonstrate that BM‐MSC is a promising therapeutic candidate against post‐stroke pneumonia, with dual functions of anti‐infection and immunol modulation, which is more than a match for antibiotics treatment.
Funder
National Natural Science Foundation of China
Subject
General Physics and Astronomy,General Engineering,Biochemistry, Genetics and Molecular Biology (miscellaneous),General Materials Science,General Chemical Engineering,Medicine (miscellaneous)
Cited by
1 articles.
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