Precise Identification of Glioblastoma Micro‐Infiltration at Cellular Resolution by Raman Spectroscopy

Author:

Zhu Lijun12ORCID,Li Jianrui3,Pan Jing3,Wu Nan4,Xu Qing3,Zhou Qing‐Qing3,Wang Qiang5,Han Dong6,Wang Ziyang6,Xu Qiang3,Liu Xiaoxue7,Guo Jingxing8,Wang Jiandong4,Zhang Zhiqiang3,Wang Yiqing6,Cai Huiming6,Li Yingjia2,Pan Hao5,Zhang Longjiang3,Chen Xiaoyuan910111213,Lu Guangming1314ORCID

Affiliation:

1. Department of Radiology, Jinling Hospital, The First School of Clinical Medicine Southern Medical University 305 Zhongshan Road East, Xuanwu Nanjing 210002 China

2. Department of Medicine Ultrasonics Nanfang Hospital Southern Medical University Guangzhou 510515 China

3. Department of Radiology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University 305 Zhongshan Road East, Xuanwu Nanjing 210002 China

4. Department of Pathology, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University Nanjing 210002 China

5. Department of Neurosurgery, Jinling Hospital, Affiliated Hospital of Medical School Nanjing University 305 Zhongshan Road East, Xuanwu Nanjing 210002 China

6. Department of Biomedical Engineering, College of Engineering and Applied Sciences, State Key Laboratory of Analytical Chemistry for Life Science Nanjing University Nanjing 210002 China

7. Department of Radiology Nanjing First Hospital Nanjing Medical University Nanjing 210002 China

8. School of Chemistry Chemical Engineering and Life Sciences Wuhan University of Technology Wuhan 430000 China

9. Departments of Diagnostic Radiology, Surgery, Chemical and Biomolecular Engineering, and Biomedical Engineering, Yong Loo Lin School of Medicine and College of Design and Engineering National University of Singapore Singapore 119074 Singapore

10. Clinical Imaging Research Centre Centre for Translational Medicine Yong Loo Lin School of Medicine National University of Singapore Singapore 117599 Singapore

11. Nanomedicine Translational Research Program, Yong Loo Lin School of Medicine National University of Singapore Singapore 117597 Singapore

12. Theranostics Center of Excellence (TCE), Yong Loo Lin School of Medicine National University of Singapore 11 Biopolis Way Helios 138667 Singapore

13. Institute of Molecular and Cell Biology, Agency for Science, Technology, and Research (A*STAR) 61 Biopolis Drive, Proteos Singapore 138673 Singapore

14. State Key Laboratory of Analytical Chemistry for Life Science School of Chemistry and Chemical Engineering Nanjing University Nanjing 210002 China

Abstract

AbstractPrecise identification of glioblastoma (GBM) microinfiltration, which is essential for achieving complete resection, remains an enormous challenge in clinical practice. Here, the study demonstrates that Raman spectroscopy effectively identifies GBM microinfiltration with cellular resolution in clinical specimens. The spectral differences between infiltrative lesions and normal brain tissues are attributed to phospholipids, nucleic acids, amino acids, and unsaturated fatty acids. These biochemical metabolites identified by Raman spectroscopy are further confirmed by spatial metabolomics. Based on differential spectra, Raman imaging resolves important morphological information relevant to GBM lesions in a label‐free manner. The area under the receiver operating characteristic curve (AUC) for Raman spectroscopy combined with machine learning in detecting infiltrative lesions exceeds 95%. Most importantly, the cancer cell threshold identified by Raman spectroscopy is as low as 3 human GBM cells per 0.01 mm2. Raman spectroscopy enables the detection of previously undetectable diffusely infiltrative cancer cells, which holds potential value in guiding complete tumor resection in GBM patients.

Funder

National Natural Science Foundation of China

Fundamental Research Funds for the Central Universities

National University of Singapore

National Medical Research Council

National Research Foundation

Publisher

Wiley

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3