Affiliation:
1. Clinical Biochemistry Department, Medical Faculty Shahrood Azad University Shahrood Iran
2. Department of Clinical Biochemistry, Faculty of Medicine Mashhad University of Medical Sciences Mashhad Iran
3. Department of Medical Laboratory Sciences Varastegan Institute for Medical Sciences Mashhad Iran
4. Department of Biology, Mashhad Branch Islamic Azad University Mashhad Iran
5. Surgical Oncology Research Center Mashhad University of Medical Sciences Mashhad Iran
Abstract
AbstractObjectivesOxidative stress is one of the carcinogenic mechanisms underlying the development of glioblastoma multiforme (GBM), a highly aggressive brain tumor type associated with poor prognosis. Curcumin is known to be an efficient antioxidant, anti‐inflammatory, and anticancer compound. However, its poor solubility in water, inappropriate pharmacokinetics, and low bioavailability limit its use as an antitumor drug. We prepared PLGA‐based curcumin nanoparticles changed with folic acid and chitosan (curcumin‐PLGA‐CS‐FA) and evaluated its effects on GBM tumor cells' redox status.MethodsThe nanoprecipitation method was used to synthesize CU nanoparticles (CU‐NPs). The size, morphology, and stability were characterized by DLS, SEM, and zeta potential analysis, respectively. The CU‐NPs' toxic properties were studied by MTT assay and measuring the intracellular reactive oxygen species (ROS) and malondialdehyde (MDA) concentrations. The study was completed by measuring the gene expression levels and activity of superoxide dismutase, catalase, glutaredoxin, and thioredoxin antioxidant enzymes.ResultsThe size, polydispersity index, and zeta potential of CU‐NPs were 77.27 nm, 0.29, and −22.45 mV, respectively. The encapsulation efficiency was approximately 98%. Intracellular ROS and MDA levels decreased after CU‐NP treatment. Meanwhile, the CU‐NPs increased gene expression and activity of superoxide dismutase, catalase, glutaredoxin, and thioredoxin antioxidant enzymes.ConclusionCU‐NPs might be effective in the prevention and treatment of glioblastoma cancer by modulating the antioxidant–oxidant balance.
Subject
Complementary and alternative medicine,Drug Discovery,Plant Science,Molecular Medicine,General Medicine,Biochemistry,Food Science,Analytical Chemistry
Cited by
2 articles.
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