Arginine‐methylated c‐Myc affects mitochondrial mitophagy in mouse acute kidney injury via Slc25a24

Author:

Liu Ying1,Liu Naiquan1,He Ping1,Cao Shiyu2,Li Huabing3,Liu Dajun1ORCID

Affiliation:

1. Department of Nephrology Shengjing Hospital of China Medical University Shenyang China

2. Grade 2018 Clinical Medicine China Medical University Shenyang China

3. Department of Nephrology Tiemei General Hospital of Liaoning Province Health Industrial Group Tieling China

Abstract

AbstractThe transcription factor methylated c‐Myc heterodimerizes with MAX to modulate gene expression, and plays an important role in energy metabolism in kidney injury but the exact mechanism remains unclear. Mitochondrial solute transporter Slc25a24 imports ATP into mitochondria and is central to energy metabolism. Gene Expression Omnibus data analysis reveals Slc25a24 and c‐Myc are consistently upregulated in all the acute kidney injury (AKI) cells. Pearson correlation analysis also shows that Slc25a24 and c‐Myc are strongly correlated ( > 0.9). Mutant arginine methylated c‐Myc (R299A and R346A) reduced its combination with MAX when compared with the wild type of c‐Myc. On the other hand, the Slc25a24 levels were also correspondingly reduced, which induced the downregulation of ATP production. The results promoted reactive oxygen species (ROS) production and mitophagy generation. The study revealed that the c‐Myc overexpression manifested the most pronounced mitochondrial DNA depletion. Additionally, the varied levels of mitochondrial proteins like TIM23, TOM20, and PINK1 in each group, particularly the elevated levels of PINK1 in AKI model groups and lower levels of TIM23 and TOM20 in the c‐Myc overexpression group, suggest potential disruptions in mitochondrial dynamics and homeostasis, indicating enhanced mitophagy or mitochondrial loss. Therefore, arginine‐methylated c‐Myc affects mouse kidney injury by regulating mitochondrial ATP and ROS, and mitophagy via Slc25a24.

Publisher

Wiley

Subject

Cell Biology,Clinical Biochemistry,Physiology

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