Comprehensive analysis of Eleutherococcus senticosus (Rupr. & Maxim.) Maxim. fruits based on UPLC–MS/MS and GC–MS: A rapid qualitative analysis

Author:

Chang Yaodan1,Jiang Yong1,Chen Jingnan1,Li Sen1,Wang Yimeng1,Chai Linlin2,Ma Jingwen3,Wang Zhibin1ORCID

Affiliation:

1. Key Laboratory of Basic and Application Research of Beiyao, Ministry of Education Heilongjiang University of Chinese Medicine Harbin China

2. Department of Rheumatism, The First Affiliated Hospital Heilongjiang University of Chinese Medicine Harbin China

3. Department of Acupuncture, The Second Affiliated Hospital Heilongjiang University of Chinese Medicine Harbin China

Abstract

AbstractEleutherococcus senticosus (Rupr. & Maxim.) Maxim. fruits (ESF), as a natural edible fruit, has long been popularized. However, few studies have conducted comprehensive chemical analyses of it. This study aimed to assess nonvolatile, volatile, and fatty oil components of ESF and to preliminarily explore the antioxidant activities. The qualitative and quantitative analyses of volatile and fatty oil components of ESF from 15 different regions were performed by the gas chromatography–mass spectrometry (GC–MS). Totally, 37 and 28 compounds were identified from volatile oil and fatty oil, respectively. The ultra‐high‐performance liquid chromatography–quadrupole time‐of‐flight mass spectrometry (UPLC–QTOF–MS/MS) was used to accurately detect 43 compounds of nonvolatile components. The volatile and fatty oil components and nonvolatile components of ESF were used as samples to determine the antioxidant activity of 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) in vitro. The components of ESF had antioxidant activity, and the nonvolatile components had stronger antioxidant activity. The results revealed that the proposed method, which is of great significance for the screening of new active ingredients, is valuable for the identification of pharmaceutical component and further development of food industry.

Publisher

Wiley

Subject

Food Science

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