A Copper Sulfide Doped 2D‐MXene‐Based Ultrasensitive Label‐Free Electrochemical Immunosensor for EpCAM Antigen Detection

Abstract

ABSTRACTHerein, a novel CuS‐anchored 2D‐Ti3C2Tx composite material was synthesized by a one‐pot hydrothermal method. This CuS/Ti3C2Tx hybrid was used to prepare a novel label‐free electrochemical immunosensor for EpCAM antigen detection. The immunosensor has been fabricated by electrophoretic deposition of CuS/Ti3C2Tx hybrid onto the ITO electrode followed by the immobilization of anti‐EpCAM. The electrochemical biosensing studies of this fabricated immunosensor, BSA/anti‐EpCAM/CuS/Ti3C2Tx@ITO demonstrated a wide linear range (0.01 fg/mL to 100 ng/mL) and low LOD (0.0057 fg/mL), together with high sensitivity (461.85 μA fg−1 mL cm−2) for EpCAM antigen detection. The clinical validation of the proposed immunosensor was conducted with serum samples. Moreover, the immunosensor was found to be reproducible, specific, selective, and stable over a long period of 42 days.