Affiliation:
1. Division of Applied Medicine, Institute of Medical Sciences University of Aberdeen Aberdeen UK
2. Student Research Committee Lorestan University of Medical Sciences Khorramabad Iran
3. USERN Office Lorestan University of Medical Sciences Khorramabad Iran
4. Department of Immunology Lorestan University of Medical Sciences Khorramabad Iran
Abstract
AbstractBackground and AimThe Duffy (FY) blood group system has six known antigens among which the Fya and Fyb are known as major antigens. Fyx phenotype forms as a result of two point mutations in the FYB allele leading to instability of Duffy protein and so reduction of Duffy antigen expression in the cells. This study aimed to investigate the FYX allele frequency in the Scottish population.MethodsThe Duffy blood group system was serologically and molecularly investigated in 222 samples collected from donors of Aberdeen Regional Blood Transfusion Center (BTC). The haemagglutination and BeadChip microarray chemistry methods were used for phenotyping and genotyping. Confirmatory tests were also used to check the discrepant results.ResultsIn this study, the frequency of Duffy blood group phenotypes including Fya+, Fya+b+, and Fyb+ were 17.57%, 42.79%, and 39.64%, respectively. Furthermore, the frequency of FYA/FYA, FYA/FYB, and FYB/FYB genotypes was estimated to be 14.41%, 45.95%, and 39.64%, respectively, using the Bioarray method. In the present study, based on Duffy DNA sequencing results, 12 samples (5.41%) had just one FYX allele.ConclusionThe frequency of the FYX allele in this study was estimated to be 0.0270% which is more than the results reported so far.
Funder
College of Life Sciences and Medicine, University of Aberdeen