Affiliation:
1. School of Nano‐Tech and Nano‐Bionics University of Science and Technology of China Hefei China
2. Suzhou Institute of Nano‐tech and Nano‐bionics Chinese Academy of Sciences Suzhou China
3. State Key Laboratory of Radiation Medicine and Protection School for Radiological and Interdisciplinary Sciences (RAD‐X) & Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions Soochow University Suzhou China
Abstract
AbstractAs the field of antibody therapeutics advances rapidly, membrane proteins, particularly G protein‐coupled receptors (GPCRs), have emerged as highly sought‐after drug targets. However, the challenges associated with extracting membrane proteins have created a demand for effective antibody screening systems targeting these proteins. In this study, we propose developing an innovative antibody screening strategy (Abplex) based on high‐content imaging. This approach leverages intact cells that express target membrane proteins, facilitating the presentation of proteins in their native conformation. Furthermore, it acquires both specific and non‐specific binding signals in a single well, thereby bolstering the robustness of the outcomes. The technique involves just one step and can be completed within 50 min, enabling the analysis of a single sample in just one second. The amalgamation of dependable experimental findings, a simplified workflow, reduced hands‐on time, and a swift analytical pace positions our method for superior throughput and precision when juxtaposed with traditional techniques such as CbELISA and FACS. Moreover, we introduce the concept of cell barcoding, wherein cells are labeled with different fluorescence spatial patterns. This feature allows for multiplexed detection to meet the needs of various experiments. The characteristics of Abplex promise to expedite GPCR‐targeting antibody discovery, advance therapeutics and enable new disease treatments.
Funder
National Natural Science Foundation of China
Subject
Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine