Temperature Down‐Shift Modifies Expression of UPR‐/ERAD‐Related Genes and Enhances Production of a Chimeric Fusion Protein in CHO Cells-Reference-Cited by-同舟云学术

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Temperature Down‐Shift Modifies Expression of UPR‐/ERAD‐Related Genes and Enhances Production of a Chimeric Fusion Protein in CHO Cells

Published:2020-04-27 Issue:2 Volume:16 Page:2000081
ISSN:1860-6768
Container-title:Biotechnology Journal
language:en
Short-container-title:Biotechnol. J.

Author:

Torres Mauro¹,Akhtar Samia¹,McKenzie Edward A.¹²,Dickson Alan J.¹^ORCID

Affiliation:

1. Manchester Institute of Biotechnology Faculty of Science and Engineering University of Manchester Manchester M1 7DN UK

2. Protein Expression Facility Manchester Institute of Biotechnology Faculty of Life Sciences University of Manchester Manchester M1 7DN UK

Funder

Biotechnology and Biological Sciences Research Council

Publisher

Wiley

Subject

Molecular Medicine,Applied Microbiology and Biotechnology,General Medicine

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/biot.202000081

Reference52 articles.

1. Biopharmaceutical benchmarks 2018

2. Advances in recombinant antibody manufacturing

3. Visualisation of intracellular production bottlenecks in suspension-adapted CHO cells producing complex biopharmaceuticals using fluorescence microscopy

4. Over‐expression of secreted proteins from mammalian cell lines

5. Model-directed engineering of “difficult-to-express” monoclonal antibody production by Chinese hamster ovary cells

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