Affiliation:
1. Institute for Advanced Materials School of Materials Science and Engineering Jiangsu University Zhenjiang 212013 China
2. Department of Medical Oncology Zhenjiang Clinical Medical College of Nanjing Medical University Jiangsu University Affiliated People's Hospital Zhenjiang 212000 China
3. College of Food Science and Engineering Yangzhou University Yangzhou 225009 China
4. School of Chemistry and Chemical Engineering Jiangsu University Zhenjiang 212013 China
5. Department of Laboratory Medicine The First Affiliated Hospital of Nanjing Medical University Nanjing 210029 China
Abstract
AbstractDeveloping a robust strategy for profiling heterogeneous circular tumor cells specifically, distinguishing the phenotypes of which in blood sample of cancer patient precisely, and releasing them sequentially, is significant for cancer management by liquid biopsy. Herein, a bio‐inspired free‐standing and flexible film composed of TiO2 nanotube and silk fibroin, fabricated with multiply dynamic bioactive surface (TSF/MDBS) by a simple and eco‐friendly way including using polydopamine chemistry and dual dynamic covalent chemistry, is reported. The as‐prepared TSF/MDBS binds specific peptides toward cells with epithelial biomarker and human epithelial growth factor receptor 2 (HER2) biomarker, and antifouling agents bovine serum albumin for obviating platelets and proteins adhering of blood, can capture heterogeneous CTCs with enhanced capability due to the cytocompatible soft film and exquisite surface design, and further release the captured cells as program, by specifically breaking down the covalent bonds in sequence via the action of adding biocompatible molecules fructose and glutathione. By applying the TSF/MDBS, it can be tailored into desired pieces for identifying CTCs with different phenotypes (HER2‐high and HER2‐low) from the unprocessed blood samples of breast cancer patients, and finally profiling these heterogeneous CTCs, to discriminate HER2 positive or negative of breast cancer patients in clinical applications.
Funder
National Natural Science Foundation of China
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science