Affiliation:
1. Division of Micro‐ and Nanosystems Department of Intelligent Systems KTH Royal Institute of Technology Malvinas Väg 10 pl 5 Stockholm SE‐10044 Sweden
2. Division of Nanobiotechnology Department of Protein Science KTH Royal Institute of Technology Tomtebodavägen 23a Stockholm SE‐17165 Sweden
3. The Rolf Luft Research center for Diabetes and Endocrinology Karolinska Institutet Stockholm SE‐17176 Sweden
4. AIMES Center for the Advancement of Integrated Medical and Engineering Sciences Department of Neuroscience Karolinska Institutet Solnavägen 9/B8 Stockholm SE‐17165 Sweden
Abstract
AbstractHybridizing biological cells with man‐made sensors enable the detection of a wide range of weak physiological responses with high specificity. The anterior chamber of the eye (ACE) is an ideal transplantation site due to its ocular immune privilege and optical transparency, which enable superior noninvasive longitudinal analyses of cells and microtissues. Engraftment of biohybrid microstructures in the ACE may, however, be affected by the pupillary response and dynamics. Here, sutureless transplantation of biohybrid microstructures, 3D printed in IP‐Visio photoresin, containing a precisely localized pancreatic islet to the ACE of mice is presented. The biohybrid microstructures allow mechanical fixation in the ACE, independent of iris dynamics. After transplantation, islets in the microstructures successfully sustain their functionality for over 20 weeks and become vascularized despite physical separation from the vessel source (iris) and immersion in a low‐viscous liquid (aqueous humor) with continuous circulation and clearance. This approach opens new perspectives in biohybrid microtissue transplantation in the ACE, advancing monitoring of microtissue–host interactions, disease modeling, treatment outcomes, and vascularization in engineered tissues.
Funder
European Research Council
Stiftelsen för Strategisk Forskning
Knut och Alice Wallenbergs Stiftelse
Wenner-Gren Stiftelserna
Novo Nordisk Fonden
Familjen Erling-Perssons Stiftelse
Berth von Kantzows Stiftelse
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Cited by
1 articles.
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