Affiliation:
1. Department of Health Sciences and Technology GAIHST Gachon University Incheon 21999 South Korea
2. Lee Gil Ya Cancer and Diabetes Institute Gachon University Incheon 21999 South Korea
3. Department of Immunology School of Medicine Jeonbuk National University Jeonju 54907 South Korea
4. Department of Physiology School of Medicine Gachon University Incheon 21999 South Korea
5. Department of Rehabilitation Medicine Eulji Hospital School of Medicine Eulji University Seoul 01830 South Korea
6. Ectosome Inc. Incheon 21999 South Korea
Abstract
AbstractCurrent clinical therapeutic efficacy for the treatment of osteo‐ and rheumatoid‐arthritis is obviously limited. Although mesenchymal stem cells (MSCs) are considered as a source of promising regenerative therapy, un‐modified or genetically engineered MSCs injected in vivo restrict their clinical utility because of the low drug efficacy and unpredicted side effect, respectively. Herein, a strategy to enhance the migration efficacy of MSCs to inflamed joints via an inflammation‐mediated education process is demonstrated. To reinforce the limited anti‐inflammatory activity of MSCs, gold nanostar loaded with triamcinolone is conjugated to MSC. Furthermore, near‐infrared laser‐assisted photothermal therapy (PTT) induced by gold nanostar significantly elevates the anti‐inflammatory efficacy of the developed drugs, even in advanced stage arthritis model. An immunological regulation mechanism study of PTT is first suggested in this study; the expression of the interleukin 22 receptor, implicated in the pathogenesis of arthritis, is downregulated in T lymphocytes by PTT, and Th17 differentiation from naïve CD4 T cell is inhibited. Collectively, inflammation‐targeting MSCs conjugated with triamcinolone‐loaded gold nanostar (Edu‐MSCs‐AuS‐TA) promote the repolarization of macrophages and decrease neutrophil recruitment in joints. In addition, Edu‐MSCs‐AuS‐TA significantly alleviate arthritis‐associated pain, improve general locomotor activity, and more importantly, induce cartilage regeneration even for severe stages of arthritis model.
Funder
National Research Foundation of Korea
Subject
Mechanical Engineering,Mechanics of Materials,General Materials Science
Cited by
1 articles.
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