Supramolecular Hydrogel with Ultra‐Rapid Cell‐Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration-Reference-Cited by-同舟云学术

Supramolecular Hydrogel with Ultra‐Rapid Cell‐Mediated Network Adaptation for Enhancing Cellular Metabolic Energetics and Tissue Regeneration

Published:2024-01-31 Issue: Volume: Page:
ISSN:0935-9648
Container-title:Advanced Materials
language:en
Short-container-title:Advanced Materials

Author:

Li Zhuo¹,Yang Boguang²,Yang Zhengmeng²,Xie Xian¹,Guo Zhengnan³,Zhao Jianyang³,Wang Ruinan³,Fu Hao³,Zhao Pengchao³,Zhao Xin⁴,Chen Guosong⁵,Li Gang²,Wei Fuxin⁶,Bian Liming⁴⁷⁸⁹^ORCID

Affiliation:

1. Department of Biomedical Engineering The Chinese University of Hong Kong Hong Kong 999077 P.R. China

2. Department of Orthopaedic and Traumatology The Chinese University of Hong Kong Hong Kong 999077 P.R. China

3. School of Biomedical Sciences and Engineering South China University of Technology Guangzhou 511442 P.R. China

4. Department of Biomedical Engineering The Hong Kong Polytechnic University Hong Kong 999077 P.R. China

5. Department of Macromolecular Science Fudan University Shanghai 200433 P.R. China

6. Department of Orthopedic Surgery The Seventh Affiliated Hospital of Sun Yat‐sen University Shenzhen 518107 P.R. China

7. National Engineering Research Center for Tissue Restoration and Reconstruction South China University of Technology Guangzhou 511442 P.R. China

8. Key Laboratory of Biomedical Materials and Engineering of the Ministry of Education South China University of Technology Guangzhou 511442 P.R. China

9. Guangdong Provincial Key Laboratory of Biomedical Engineering South China University of Technology Guangzhou 511442 P.R. China

Abstract

AbstractCellular energetics plays an important role in tissue regeneration, and the enhanced metabolic activity of delivered stem cells can accelerate tissue repair and regeneration. However, conventional hydrogels with limited network cell adaptability restrict cell–cell interactions and cell metabolic activities. In this work, we showed that a cell‐adaptable hydrogel with high network dynamics enhanced the glucose uptake and fatty acid β‐oxidation (FAO) of encapsulated human mesenchymal stem cells (hMSCs) compared with a hydrogel with low network dynamics. We further showed that the hMSCs encapsulated in the dynamic hydrogels exhibited increased TCA cycle activity, oxidative phosphorylation (OXPHOS) and ATP biosynthesis via an E‐cadherin‐ and AMPK‐dependent mechanism. The in vivo evaluation further showed that the delivery of MSCs by the dynamic hydrogel enhanced in situ bone regeneration in an animal model. We believe that our findings provide critical insights into the impact of stem cell–biomaterial interactions on cellular metabolic energetics and the underlying mechanisms.This article is protected by copyright. All rights reserved

Publisher

Wiley

Subject

Mechanical Engineering,Mechanics of Materials,General Materials Science

Link

https://onlinelibrary.wiley.com/doi/pdf/10.1002/adma.202307176