Simultaneous determination of main taxoids in Taxus needles extracts by solid‐phase extraction‐high‐performance liquid chromatography with pentafluorophenyl column

Abstract

AbstractA simple and accurate RP‐HPLC method with pentafluorophenyl (PFP) column was developed for the simultaneous determination of six taxoids, i.e. paclitaxel, 10‐deacetylbaccatin III (10‐DAB III), 7‐xylosyl‐10‐deacetyltaxol (7‐xyl‐10‐DAT), 10‐deacetyltaxol (10‐DAT), cephalomannine and 7‐epi‐10‐deacetyltaxol (7‐epi‐10‐DAT), in the extracts from the needles of three Taxus species. The mobile phase consisted of acetonitrile (A) and water (B), and the extracts were separated using gradient elution program: 30% A at the first 7 min, and then ramped to 42% A at 8 min, held until 38 min. The developed method was validated with satisfactory precision (RSD < 2.61%), repeatability (RSD < 2.92%) and recovery (95.19–104.47%). The above taxoids in the extracts of Taxus cuspidata, T. chinensis and T. media were analyzed with the developed RP‐HPLC method, and the results showed that the contents of different taxoids in three mentioned species were distinct. Maximal amounts of 10‐DAB III, 7‐xyl‐10‐DAT and 7‐epi‐10‐DAT appeared in T. chinensis, while T. media possessed the highest content of 10‐DAT, cephalomannine and paclitaxel. The developed method is accurate and efficient. It can be reliably used in the improved determination of taxoids for the quality control of Taxus species. Copyright © 2008 John Wiley & Sons, Ltd.