Scoparone inhibits the development of hepatocellular carcinoma by modulating the p38 MAPK/Akt/NF‐κB signaling in nonalcoholic fatty liver disease mice

Author:

Ye Miaoqing1,Liu Chunyan2,Liu Jiaojiao1,Lu Fenping3,Xue Jingdong,Li Fenping1,Tang Yinghui1ORCID

Affiliation:

1. Department of Liver Disease Shaanxi Provincial Hospital of Chinese Medicine Xi'an China

2. First Clinical Medical College Shaanxi University of Chinese Medicine Xian yang China

3. Literature research institute Shaanxi Academy of Traditional Chinese Medicine Xi'an China

Abstract

AbstractBackground and study aimThe mechanisms underlying the progression of non‐alcoholic fatty liver disease (NAFLD) into hepatocellular carcinoma (HCC) remains confusing and the therapeutics approaches are also challenging. Here, we aimed to investigate the effects of scoparone on the treatment of HCC stemmed from NAFLD and the underlying mechanisms.Materials and methodsA model of NAFLD‐HCC was created in mice, and these mice were treated with scoparone. Biochemical assays were conducted to assess the levels of biochemical markers. Tumors were evaluated through morphological examination. Histopathological analyses were performed using oil red O, Hematoxylin and Eosin, and Masson coloration assays. Immunohistochemistry (IHC) and RT‐PCR were performed to analyze protein expression and measure mRNA expression levels, respectively.ResultsScoparone could ameliorate the pathological alterations observed in NAFLD‐HCC mouse model. IHC analysis indicated an upregulation of NF‐κB p65 expression in both NAFLD and NAFLD‐HCC models, which was subsequently reverted by scoparone administration. Furthermore, scoparone treatment resulted in a reversal of the increased mRNA expression levels of NF‐κB target genes, including TNF‐α, MCP‐1, iNOS, COX‐2, NF‐κB, and MMP‐9, which were originally elevated in the NAFLD‐HCC condition. Additionally, scoparone exhibited a capacity to counteract the activation of the MAPK/Akt signaling in the NAFLD‐HCC model.ConclusionThese findings suggest that scoparone holds promise as a potential therapeutic agent for NAFLD‐associated HCC, and its model of action may involve the regulation of inflammatory pathways governed by the MAPK/Akt/NF‐κB signaling cascade.

Funder

Natural Science Foundation of Shaanxi Province

Publisher

Wiley

Subject

Health, Toxicology and Mutagenesis,Management, Monitoring, Policy and Law,Toxicology,General Medicine

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