Affiliation:
1. Laboratory of Cellular and Molecular Neurobiology School of Life Sciences Jawaharlal Nehru University New Delhi 110067 India
2. Department of Computational Biology Indraprastha Institute of Information Technology New Delhi 110067 India
Abstract
BackgroundAlzheimer's disease (AD) is the most prevalent dementia, affecting a large number of populations. Despite being under scrutiny for decades, an effective therapeutic option is still not available.Methods and resultsThis study explores the therapeutic role of a nootropic herb Bacopa monnieri (BM) in AD‐like pathological conditions produced by injecting preformed amyloid‐β42 (Aβ42) fibril bilaterally into hippocampus of Wistar rats, and ethanolic extract of BM is orally administered for 4 weeks. Assessment of behavioral changes reveals that BM treatment ameliorates Aβ42‐induced cognitive impairment and compromised explorative behavior. Supplementation of BM also reduces oxidative stress biomarkers, proinflammatory cytokines, and cholinesterase activity in the AD rats. Additionally, BM treatment restores Bcl‐2‐associated X protein (Bax)/ B‐cell lymphoma 2 (Bcl‐2) imbalance, increases neurotrophic factors expression, and prevents neurodegeneration validated by quantifying Nissl‐positive hippocampal neurons. Interestingly, BM administration eliminates amyloid plaques in the hippocampal region and normalizes the Aβ42‐induced increase in phospho‐tau and total tau expression. Mechanistic investigations reveal that BM interacts with glycogen synthase kinase (GSK‐3β) and restores Wnt/β‐catenin signaling.ConclusionBM has been used in diet as a nootropic herb for several centuries. This study highlights the anti‐Alzheimer activity of BM from the behavioral to the molecular level by modulating mitochondrial dysfunction, and GSK‐3β mediates the Wnt/β‐catenin signaling pathway.
Funder
Department of Biotechnology, Ministry of Science and Technology, India
Subject
Food Science,Biotechnology