Inulin Supplementation Modulates the Hepatic Transcriptome, Metabolome, and Ferritin Content in Ovariectomized Rats

Author:

Zhao Xiaorui1,He Weiwei12ORCID,Jakobsen Louise M. A.1ORCID,Zachariassen Line F.3,Hansen Axel K.3,Rasmussen Martin Krøyer1ORCID,Bertram Hanne Christine1ORCID

Affiliation:

1. Department of Food Science Aarhus University Agro Food Park 48, DK‐8200 Aarhus N Denmark

2. State Key Laboratory of Food Science and Resources Nanchang University Nanjing Road E. 235 Nanchang 330047 China

3. Department of Veterinary and Animal Sciences Faculty of Health and Medical Sciences University of Copenhagen Ridebanevej 9 Frederiksberg C 1870 Denmark

Abstract

ScopeLiver is an important metabolic organ regulating whole‐body homeostasis. This study aims to investigate how prebiotic‐induced changes in the metabolic activity of the gut microbiome (GM) and dietary calcium depletion modulates the hepatic metabolome and transcriptome.Methods and resultsThe serum metabolome, liver metabolome, and transcriptome are determined on samples from ovariectomized (OVX) rats fed a control diet (Control, n = 7), a control diet supplemented with 5% w/w inulin (Inulin, n = 7), or a calcium‐deficient diet (CaDef, n = 7). Inulin fortification is associated with higher serum concentrations of acetate, 3‐hydroxybutyrate, and reduced concentration of dimethyl sulfone, revealing that changes in the metabolic activity of the GM are reflected in circulating metabolites. Metabolomics also reveal that the inulin‐fortified diet results in lower concentrations of hepatic glutamate, serine, and hypoxanthine while transcriptomics reveal accompanying effects on the hepatic expression of ferric iron binding‐related genes. Inulin fortification also induces effects on the hepatic expression of genes involved in olfactory transduction, suggesting that prebiotics regulate liver function through yet unidentified mechanisms involving olfactory receptors.ConclusionInulin ingestion impacts hepatic gene expression and is associated with an upregulation of ferritin synthesis‐related genes and liver ferritin content.

Funder

Mejeribrugets ForskningsFond

Chinese Government Scholarship

Publisher

Wiley

Subject

Food Science,Biotechnology

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