Combination of chemical profiling and network pharmacology analysis to investigate the potential mechanism of Li‐Zhong‐Xiao‐Pi granules in the treatment of gastric precancerous lesions

Author:

Liu Chenchen1,Chen Huiling1,Zhang Yida2,Li Meng3,Jiang Qiyao1,Wang Zhendong1,Yu Liangwen1,Wang Qi1,Pan Huafeng1,Zhuo Yue1ORCID

Affiliation:

1. Science and Technology Innovation Center Guangzhou University of Chinese Medicine Guangzhou China

2. Department of Allergy and Clinical Immunology, National Center for Respiratory Medicine, National Clinical Research Center for Respiratory Disease, State Key Laboratory of Respiratory Disease, Guangzhou Institute of Respiratory Health The First Affiliated Hospital of Guangzhou Medical University Guangzhou China

3. Department of Pediatric Surgery, Guangzhou Institute of Pediatrics, Guangdong Provincial Key Laboratory of Research in Structural Birth Defect Disease, Guangzhou Women and Children’s Medical Center Guangzhou Medical University Guangzhou China

Abstract

AbstractLi‐Zhong‐Xiao‐Pi granules (LZXP) are effective for treating gastric precancerous lesions (GPL) in traditional Chinese medicine. However, the active compounds of LZXP and their potential therapeutic mechanism in GPL remained unclarified. The purpose of this study is to investigate the chemical composition and potential targets of LZXP. Based on the accurate masses, ion fragments, and literature data, a total of 128 compounds were identified in the LZXP sample using ultra‐performance liquid chromatography and quadrupole time‐of‐flight mass spectrometry (UPLC–Q‐TOF–MS) in both positive and negative ion modes, and 28 of these compounds were exactly determined by comparison with authentic reference standards. Meanwhile, 11 typical components were quantified via UPLC during a 24 min period. The linearity, accuracy, stability and recovery of the method were all proven. Through the network pharmacological analysis, six chemicals (quercetin, 4′‐hydroxywogonin, sinensetin, 5, 7, 8, 3′, 4′‐pentamethoxyflavanone, 8‐gingerdione and quercetin) were identified as the active ingredients, and five LZXP targets (AKT1, CYP1B1, PTGS2, MMP9 and EGFR) were found to be the crucial molecules in the treatment of GPL. This study provides a systematic and applicable method for the rapid screening and identification of the chemical constituents from LZXP, and an effective understanding the mechanism of LZXP in the treatment of GPL.

Funder

Basic and Applied Basic Research Foundation of Guangdong Province

Publisher

Wiley

Subject

Clinical Biochemistry,Drug Discovery,Pharmacology,Molecular Biology,General Medicine,Biochemistry,Analytical Chemistry

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