Engineering Modular, Oxygen‐Generating Microbeads for the In Situ Mitigation of Cellular Hypoxia

Author:

Accolla Robert P.1,Liang Jia‐Pu1,Lansberry Taylor R.1,Miravet Irayme Labrada1,Loaisiga Marlon1,Sardi Baltasar Lopez1,Stabler Cherie L.123ORCID

Affiliation:

1. J. Crayton Pruitt Family Department of Biomedical Engineering University of Florida Gainesville FL 32610 USA

2. Department of Immunology and Pathology College of Medicine University of Florida Gainesville FL 32610 USA

3. University of Florida Diabetes Institute Gainesville FL 32610 USA

Abstract

AbstractInsufficient oxygenation is a key obstacle in the design of clinically scalable tissue‐engineered grafts. In this work, an oxygen‐generating composite material, termed OxySite, is created through the encapsulation of calcium peroxide (CaO2) within polydimethylsiloxane and formulated into microbeads for ease in tissue integration. Key material parameters of reactant loading, porogen addition, microbead size, and an outer rate‐limiting layer are modulated to characterize oxygen generation kinetics and their suitability for cellular applications. In silico models are developed to predict the local impact of different OxySite microbead formulations on oxygen availability within an idealized cellular implant. Promising OxySite microbead variants are subsequently coencapsulated with murine β‐cells within macroencapsulation devices, resulting in improved cellular metabolic activity and function under hypoxic conditions when compared to controls. Additionally, the coinjection of optimized OxySite microbeads with murine pancreatic islets within a confined transplant site demonstrates ease of integration and improved primary cell function. These works highlight the broad translatability delivered by this new oxygen‐generating biomaterial format, whereby the modularity of the material provides customization of the oxygen source to the specific needs of the cellular implant.

Funder

Juvenile Diabetes Research Foundation International

University of Florida

Juvenile Diabetes Research Foundation United States of America

Publisher

Wiley

Subject

Pharmaceutical Science,Biomedical Engineering,Biomaterials

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