Affiliation:
1. Postgraduate Training Base of Jinzhou Medical University in Shanghai Sixth People's Hospital Jinzhou Medical University Jinzhou 121001 China
2. Department of Orthopaedics Shanghai Sixth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine Shanghai 200233 P. R. China
3. School of Materials Science and Engineering Tongji University Shanghai 201804 China
4. Department of Orthopedics Shanghai Tenth People's Hospital School of Medicine Tongji University Shanghai 200072 China
Abstract
AbstractDiabetic wounds are a prevalent and devastating complication of diabetes, which may impede their healing and regeneration. In diabetic wounds, excess reactive oxygen species (ROS) activate the nuclear factor kappa‐B pathway, leading to transcriptional silencing of nuclear factor erythroid 2‐related factor 2 (Nrf2), resulting in a vicious cycle of oxidative stress and inflammation. Conventional nanozymes have limitations in preventing the continuous production of ROS, including the most oxidizing reactive hydroxyl radical (·OH), although they can remove preexisting ROS. Herein, a novel antioxidant nanoplatform addresses this challenge by incorporating JSH‐23 into the mesoporous of cupric‐doped cerium oxide nanozymes. Additionally, for rapid wound adaptability and durable tissue adhesion, a nanozyme hydrogel spray consisting of oxidized sodium alginate and methacrylate gelatin was constructed, named OG@CCJs. This platform resurrected Nrf2 transcriptional activity of macrophages in vitro, curbing the production of ROS at its source, particularly ·OH, while enabling the nanozymes to scavenge previously generated ROS. OG@CCJs significantly alleviated oxidative stress in diabetic wounds in vivo, promoting wound healing. Overall, the proposed nanozyme‐hydrogel spray with enhanced ·OH‐scavenging activity uses a “two‐track” antioxidant strategy to rebuild the antioxidant defense barrier of macrophages. This pioneering approach highlights the tremendous potential of OG@CCJs for facilitating diabetic wound healing.This article is protected by copyright. All rights reserved
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials