Synthetic Self‐Adjuvanted Lipopeptide Vaccines Conferred Protection against Helicobacter pylori Infection

Author:

Xue Ruo‐Yi1,Liu Chang1,Wang Jia‐Qi2,Deng Yan1,Feng Rang1,Li Guo‐Cheng1,Liu Jing‐Yi1,Cheng Hao1,Shan Zhang Shan‐1,Duan Hao1,Jin Zhe1,Zou Quan‐Ming1,Li Hai‐Bo1ORCID

Affiliation:

1. National Engineering Research Center of Immunological Products Department of Microbiology and Biochemical Pharmacy College of Pharmacy Third Military Medical University Chongqing 400038 P. R. China

2. Laboratory of Stem Cell and Developmental Biology Department of Histology and Embryology Third Military Medical University Chongqing 400038 P. R. China

Abstract

AbstractHelicobacter pylori (H. pylori) colonizes the stomach epithelium of half the world's population and is responsible for various digestive diseases and even stomach cancer. Vaccine‐mediated protection against H. pylori infection depends primarily on the specific mucosal and T‐cell responses. In this study, the synthetic lipopeptide vaccines, Hp4 (Pam2Cys modified UreB T‐cell epitope) and Hp10 (Pam2Cys modified CagA T/B cell combined epitope), not only induce the bone marrow derived dendritic cells (BMDCs) maturation by activating a variety of pattern‐recognition receptors (PRRs) such as Toll‐like receptor (TLR), Nod‐like receptor (NLR), and retinoic acid‐inducing gene (RIG) I‐like receptor (RLR), and but also stimulate BMDCs to secret cytokines that have the potential to modulate T‐cell activation and differentiation. Although intranasal immunization with Hp4 or Hp10 elicits robust epitope‐specific T‐cell responses in mice, only Hp10 confers protection against H. pylori infection, possibly due to the fact that Hp10 also induces substantial specific sIgA response at mucosal sites. Interestingly, Hp4 elevates the protective response against H. pylori infection of Hp10 when administrated in combination, characterized by better protective effect and enhanced specific T‐cell and mucosal antibody responses. The results suggest that synthetic lipopeptide vaccines based on the epitopes derived from the protective antigens are promising candidates for protection against H. pylori infection.

Publisher

Wiley

Subject

Pharmaceutical Science,Biomedical Engineering,Biomaterials

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