Affiliation:
1. Stomatology Hospital School of Stomatology Zhejiang University School of Medicine Zhejiang Provincial Clinical Research Center for Oral Diseases Key Laboratory of Oral Biomedical Research of Zhejiang Province Cancer Center of Zhejiang University 395 Yan'an road Hangzhou Zhejiang 310000 China
2. Department of Stomatology The First Affiliated Hospital of Ningbo University 59 Liuting street Ningbo Zhejiang 315000 China
3. The Third Affiliated Hospital of Wenzhou Medical University (Ruian People's Hospital) 168 Ruifeng Avenue Wenzhou Zhejiang 325016 China
Abstract
AbstractPatterned interfaces are widely used for surface modification of biomaterials because of a morphological unit similar to that of native tissue. However, engineering fast and cost‐effective high‐resolution micropatterns directly onto titanium surfaces remains a grand challenge. Herein, a simply designed ultraviolet (UV) light‐based micropattern printing to obtain geometrical patterns on implant interfaces is fabricated by utilizing customized photomasks and titanium dioxide (TiO2) nanorods as a photo‐responsive platform. The technique manipulates the cytoskeleton of micropatterning cells on the surface of TiO2 nanorods. The linear pattern surface shows the elongated morphology and parallel linear arrangements of human mesenchymal stem cells (hMSCs), significantly enhancing their osteogenic differentiation. In addition to the upregulated expression of key osteo‐specific function genes in vitro, the accelerated osseointegration between the implant and the host bone is obtained in vivo. Further investigation indicates that the developed linear pattern surface has an outstanding effect on the cytoskeletal system, and finally activates Yes‐Associated Protein (YAP)‐mediated mechanotransduction pathways, initiating hMSCs osteogenic differentiation. This study not only offers a microfabrication method that can be extended to fabricate various shape‐ and size‐controlled micropatterns on titanium surfaces, but also provides insight into the surface structure design for enhanced bone regeneration.
Funder
National Natural Science Foundation of China
National Key Research and Development Program of China
Medical Technology and Education of Zhejiang Province of China
Subject
Pharmaceutical Science,Biomedical Engineering,Biomaterials