Affiliation:
1. National Key Laboratory of Agricultural Microbiology College of Life Science and Technology Huazhong Agricultural University No. 1 Shizishan Street Wuhan 430070 P. R. China
2. Department of Chemical and Biomolecular Engineering National University of Singapore 4 Engineering Drive 4 Singapore 117585 Singapore
3. Institute of Chemistry Technische Universität Berlin Müller-Breslau-Str. 10 10623 Berlin Germany
4. Department of Biotechnology and Enzyme Catalysis Institute of Biochemistry University of Greifswald Felix Hausdorff-Str. 4 17489 Greifswald Germany
Abstract
AbstractProduction of commodity chemicals, such as benzene, toluene, ethylbenzene, and xylenes (BTEX), from renewable resources is key for a sustainable society. Biocatalysis enables one‐pot multistep transformation of bioresources under mild conditions, yet it is often limited to biochemicals. Herein, we developed a non‐natural three‐enzyme cascade for one‐pot conversion of biobased l‐phenylalanine into ethylbenzene. The key rate‐limiting photodecarboxylase was subjected to structure‐guided semirational engineering, and a triple mutant CvFAP(Y466T/P460A/G462I) was obtained with a 6.3‐fold higher productivity. With this improved photodecarboxylase, an optimized two‐cell sequential process was developed to convert l‐phenylalanine into ethylbenzene with 82 % conversion. The cascade reaction was integrated with fermentation to achieve the one‐pot bioproduction of ethylbenzene from biobased glycerol, demonstrating the potential of cascade biocatalysis plus enzyme engineering for the production of biobased commodity chemicals.
Funder
National Natural Science Foundation of China
Natural Science Foundation of Hubei Province
Fundamental Research Funds for the Central Universities
Alexander von Humboldt-Stiftung
H2020 European Research Council
Subject
General Chemistry,Catalysis
Cited by
2 articles.
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