Affiliation:
1. Department of Health Sciences and Technology ETH Zürich Schmelzbergstrasse 9 8092 Zürich Switzerland
2. TBI Université de Toulouse, CNRS, INRAE, INSA 135 Av. de Rangueil 31400 Toulouse France
3. Department of Materials ETH Zürich Wolfgang-Pauli-Strasse 10 8093 Zürich Switzerland
Abstract
AbstractSoft nanoconfinement can increase chemical reactivity in nature and has therefore led to considerable interest in transferring this universal feature to artificial biological systems. However, little is known about the underlying principles of soft nanoconfinement responsible for the enhancement of biochemical reactions. Herein we demonstrate how enzymatic polymerization can be expanded, optimized, and engineered when carried out under soft nanoconfinement mediated by lipidic mesophases. By systematically varying the water content in the mesophase and thus the diameter of the confined water nanochannels, we show higher efficiency, turnover rate, and degrees of polymerization as compared to the bulk aqueous solution, all controlled by soft nanoconfinement effects. Furthermore, we exploit the unique properties of unfreezing soft nanoconfined water to perform the first enzymatic polymerization at −20 °C in pure aqueous media. These results underpin lipidic mesophases as a versatile host system for chemical reactions and promote them as an original and unexplored platform for enzymatic polymerization.
Funder
Schweizerischer Nationalfonds zur Förderung der Wissenschaftlichen Forschung
Subject
General Chemistry,Catalysis
Cited by
1 articles.
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