An undergraduate laboratory experience using CRISPR-cas9 technology to deactivate green fluorescent protein expression in Escherichia coli
Author:
Affiliation:
1. Department of Biology; Rollins College; Winter Park Florida
2. Department of Biology; Stetson University; DeLand Florida
Funder
New England Biolabs
Publisher
Wiley
Subject
Molecular Biology,Biochemistry
Link
http://onlinelibrary.wiley.com/wol1/doi/10.1002/bmb.21206/fullpdf
Reference22 articles.
1. CRISPR provides acquired resistance against viruses in prokaryotes;Barrangou;Science,2007
2. A programmable dual-RNA-guided DNA endonuclease in adaptive bacterial immunity;Jinek;Science,2012
3. CRISPR CUREs: Running with Molecular Scissors in the Classroom. Biochem;Siniscalco;Modern Appl.,2018
4. Molecular biology at the cutting edge: A review on CRISPR/CAS9 gene editing for undergraduates;Thurtle-Schmidt;Biochem. Mol. Biol. Educ.,2018
5. CRISPR-Cas technology in and out of the classroom;Dahlberg;CRISPR J.,2018
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