Two-step ion-exchange chromatographic purification combined with reversed-phase chromatography to isolate C-peptide for mass spectrometric analysis

Author:

Kabytaev Kuanysh1,Durairaj Anita1,Shin Dmitriy1,Rohlfing Curt L.1,Connolly Shawn1,Little Randie R.1,Stoyanov Alexander V.1

Affiliation:

1. University of Missouri; School of Medicine; One Hospital Drive Columbia MO USA

Publisher

Wiley

Subject

Filtration and Separation,Analytical Chemistry

Reference21 articles.

1. An Automated On-Line Multidimensional HPLC System for Protein and Peptide Mapping with Integrated Sample Preparation

2. Henry , R. Principles of multidimensional HPLC and advantages for protein and peptide analysis. Lecture L-5 at 23rd International Symposium on the Separation of Proteins, Peptides and Polynucleotides (ICPPP) Delray Beach FL, USA

3. IEF-based multidimensional applications in proteomics: Toward higher resolution

4. Heterogeneity of Circulating Human C-peptide

5. Proinsulin and the Biosynthesis of Insulin

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