Affiliation:
1. Instituto de Química Universidade Estadual de Campinas UNICAMP Campinas São Paulo Brazil
2. Instituto Nacional de Ciência e Tecnologia de Bioanalítica Campinas São Paulo Brazil
Abstract
AbstractAn analytical method based on capillary electrophoresis (CE) using capacitively coupled contactless conductivity detection (C4D) was developed and validated for fast, straightforward, and reliable determination of lactate in artificial and human sweat samples. The background electrolyte was composed of equimolar concentrations (10 mmol/L) of 2‐(N‐morpholino)ethanesulfonic acid and histidine, with 0.2 mmol/L of cetyltrimethylammonium bromide as electroosmotic flow inverter. The limit of detection and quantification were 3.1 and 10.3 µmol/L, respectively. Recoveries in the 97 to 118% range were obtained using sweat samples spiked with lactate at three concentration levels, indicating an acceptable accuracy. The intraday and interday precisions were 1.49 and 7.08%, respectively. The proposed CE–C4D method can be a starting point for monitoring lactate concentrations in sweat samples for diagnostics, physiological studies, and sports performance assessment applications.
Funder
Conselho Nacional de Desenvolvimento Científico e Tecnológico
Fundação de Amparo à Pesquisa e ao Desenvolvimento Científico e Tecnológico do Maranhão
Fundação de Amparo à Pesquisa do Estado de São Paulo
Subject
Clinical Biochemistry,Biochemistry,Analytical Chemistry