Affiliation:
1. Institute of Food Science and Technology Federal University of Rio Grande do Sul (UFRGS) Porto Alegre RS Brazil Phone
2. Campus Uruguaiana Federal University of Pampa (Unipampa) Uruguaiana Brazil
3. Institute of Basic Health Sciences Postgraduate Program in Biological Sciences: Biochemistry Federal University of Rio Grande do Sul (UFRGS) Porto Alegre RS Brazil
Abstract
AbstractMicrocystin‐LR (MIC–LR) is a toxin which the mechanism of intoxication involves oxidative stress. Urolithin A (URO‐A) is a metabolic product from the colonic fermentation of ellagic acid with antioxidant potential. This study aimed to evaluate the putative protective effect of URO‐A against MIC–LR toxicity in C6 cells. C6 cells were incubated with MIC–LR (1 and 10 μM) and/or URO‐A (3, 30, 60 and 100 μM) for 24 h. MIC–LR induced reactive species (RS) generation, depletion of total thiol (SH) groups, and survival loss when compared with the control group. Also, at 10 μM, MIC–LR induced CAT activity inhibition. URO‐A caused CAT activity inhibition and showed a trend to increase RS generation (60 and 100 μM) per se. URO‐A at 3 μM completely attenuated the RS generation and the impairment in SH groups caused by MIC–LR. Our results demonstrated that URO‐A might offer a protective effect against toxicity caused by MIC–LR in glial cells by restoring the levels of RS and thiol groups.