Affiliation:
1. Department of Biomedical Engineering Chang Gung University Taoyuan 33302 Taiwan
2. Department of Radiation Oncology Chang Gung Memorial Hospital Linkou 33305 Taiwan
3. Department of Electrical & Electronic Engineering Yonsei University Seoul 03722 South Korea
Abstract
AbstractWhen tumor cells are released from a primary tumor into the bloodstream or lymphatic circulation system, they are exposed to a continuous shear flow environment. This environment exerts physical stresses on the tumor cells, which can activate apoptotic pathways. However, certain tumor cells have the ability to adapt to these mechanical stresses, enhancing their likelihood of survival and promoting metastasis. In this study, these tumor cells survived from shear flow environment are examined and revealed to closely link to stem cell‐like characteristics. Higher gene expression levels of self‐renewal and differentiation markers and enhanced abilities of migration, spheroid formation, and colony formation are shown. Moreover, the interaction between immune cells and the surviving cells is investigated. The results show that the surviving cells possess immune escape capabilities, implying their ability to evade immune surveillance. Additionally, these surviving cells display characteristics reminiscent of stem cells. This study holds great importance in advancing the understanding of tumor biology. By comprehending the behavior and properties of these surviving cells, new therapeutic strategies can be developed to specifically target circulating tumor cells (CTCs) and enhance cancer treatment outcomes.
Funder
Chang Gung Memorial Hospital, Linkou