Affiliation:
1. Süleyman Demirel Üniversitesi
2. SULEYMAN DEMIREL UNIVERSITY
3. Özel Meddem Hastanesi
4. MEHMET AKİF ERSOY ÜNİVERSİTESİ
Abstract
Objective
Cerebrovascular accident due to ischemia (IS)
mediated by atherosclerotic plaque in the brain
can trigger inflammation in the cerebral cortex,
hippocampus and cerebellum tissues. Radiofrequency
electromagnetic field (RF-EMF) and pulsed magnetic
field (PMF) applications can increase nitric oxide
formation from the vascular endothelial layer. The
aim of this preliminary study is to reduce the damage
caused by IS in different tissues of the brain by
magnetic field applications.
Material and Method
A total of 9 rats, one rat in each group; sham,
prophylactic RF, PMF, RF+PMF and therapeutic
RF-EMF, PMF, RF-EMF+PMF, prophylactic and
therapeutic RF-EMF+PMF and IS-only groups were
distinguished. In single or combined applications of
prophylactic/therapeutic RF-EMF and PMF groups,
rats were taken to the experimental unit for 30
minutes of magnetic field exposure before and after
30 minutes of carotid artery occlusion for IS purposes.
Histopathological hematoxylin-eosin staining in
brain tissue (cerebral cortex and hippocampus)
and cerebellum tissues taken after sacrification;
With immunohistochemical analysis, brain derived
neurotrophic factor (BDNF), tumor necrosis factoralpha
(TNF-α), mammalian target of rapamycin
(mTOR) and inducible nitric oxide synthase (iNOS)
expressions were examined.
Results
Histopathologically significant hyperemia, edema,
bleeding and neuronal degeneration were detected
in the IS group. Additionally, immunohistochemically,
an increase in TNF-α, mTOR, iNOS and a decrease
in BDNF staining were observed. Prophylactic and/or
therapeutic RF-EMF and/or PMF applications reversed
all these parameters. The greatest improvement
was observed in the Prophylactic+Therapeutic RFEMF+
PMF group.
Conclusion
As a result, the regression of IS-related inflammation in
both brain tissue parts and cerebellar tissues with RFEMF
and PMF is important in terms of the formation
of neurological deficits, the continuity of learning
and memory mechanisms, and the preservation of
balance functions.
Funder
Scientific Research Fund of Suleyman Demirel University
Publisher
Medical Journal of Suleyman Demirel University
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