Electrospun Gelatin/β-TCP Composite Nanofibers Enhance Osteogenic Differentiation of BMSCs andIn VivoBone Formation by Activating Ca2+-Sensing Receptor Signaling

Author:

Zhang Xuehui12,Meng Song13,Huang Ying1,Xu Mingming1,He Ying1,Lin Hong2,Han Jianmin2,Chai Yuan2,Wei Yan1,Deng Xuliang145ORCID

Affiliation:

1. Department of Geriatric Dentistry, Peking University School and Hospital of Stomatology, Beijing 100081, China

2. Department of Dental Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

3. Department of Prosthodontics, Peking University School and Hospital of Stomatology, Beijing 100081, China

4. National Engineering Laboratory for Digital and Material Technology of Stomatology, Beijing 100081, China

5. Beijing Laboratory of Biomedical Materials, Peking University School and Hospital of Stomatology, Beijing 100081, China

Abstract

Calcium phosphate- (CaP-) based composite scaffolds have been used extensively for the bone regeneration in bone tissue engineering. Previously, we developed a biomimetic composite nanofibrous membrane of gelatin/β-tricalcium phosphate (TCP) and confirmed their biological activityin vitroand bone regenerationin vivo. However, how these composite nanofibers promote the osteogenic differentiation of bone marrow mesenchymal stem cells (BMSCs) is unknown. Here, gelatin/β-TCP composite nanofibers were fabricated by incorporating 20 wt%β-TCP nanoparticles into electrospun gelatin nanofibers. Electron microscopy showed that the compositeβ-TCP nanofibers had a nonwoven structure with a porous network and a rough surface. Spectral analyses confirmed the presence and chemical stability of theβ-TCP and gelatin components. Compared with pure gelatin nanofibers, gelatin/β-TCP composite nanofibers caused increased cell attachment, proliferation, alkaline phosphatase activity, and osteogenic gene expression in rat BMSCs. Interestingly, the expression level of the calcium-sensing receptor (CaSR) was significantly higher on the composite nanofibrous scaffolds than on pure gelatin. For rat calvarial critical sized defects, more extensive osteogenesis and neovascularization occurred in the composite scaffolds group compared with the gelatin group. Thus, gelatin/β-TCP composite scaffolds promote osteogenic differentiation of BMSCsin vitroand bone regenerationin vivoby activating Ca2+-sensing receptor signaling.

Funder

National Basic Research Program of China

Publisher

Hindawi Limited

Subject

Cell Biology,Molecular Biology

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