G-Protein-Coupled Receptor Kinase-Interacting Protein 1 (GIT1) Promotes Head and Neck Squamous Cell Carcinoma Metastases via Activating the PI3K/AKT/mTOR Signal Pathway

Author:

Xu Runze1,Xu Ran2,Wang Yuanxiang3,Wang Weixing2,Jiang Lingling4,Gong Shishun5ORCID

Affiliation:

1. Graduate School, Shanghai Jiaotong University School of Medicine, Shanghai 200025, China

2. Centre of Cancer Diagnosis and Treatment, JiangSu Rudong County People’s Hospital, Nantong 226400, China

3. Department of Obstetrics and Gynecology, JiangSu Rudong Country People’s Hospital, Nantong 226400, China

4. Department of Medical Nursing, JiangSu Rudong Country People’s Hospital, Nantong 226400, China

5. Department of Rehabilitation, Hainan Medical College, Haikou, 571199 Hainan, China

Abstract

Objective. GIT1 is identified as a novel tumor oncogene in breast cancer. In this article, we aimed to explore the role of GIT1 in the progression of head and neck squamous cell carcinoma (HNSCC). Methods. GIT1 expression in HNSCC was detected by RT-qPCR, immunohistochemistry assay, and Western blot. HNSCC cell proliferation, migration, and invasion were examined by CCK-8 assay, Wound healing assay, and Transwell assay, respectively. Cell apoptosis was detected by flow cytometric analysis. Results. In our study, GIT1 was notably upregulated in HNSCC tissues and cells. Moreover, GIT1 expression level had positive corelation with pathological grade and nodal status of HNSCC. Functional experiments showed that knockdown of GIT1 restrained HNSCC proliferation, invasion, migration, and EMT and facilitated cell apoptosis. Furthermore, GIT1 knockdown was found to restrain HNSCC tumor growth and lung metastasis. Additionally, PI3K/AKT/mTOR signal pathway inhibitors suppressed the effect of GIT1 on HNSCC cell progression. Conclusion. GIT1 was upregulated in HNSCC and facilitated HNSCC cell progression by inducing PI3K/AKT/mTOR signal pathway. Therefore, we suggested that GIT1 might be a potential target for HNSCC treatment.

Publisher

Hindawi Limited

Subject

Applied Mathematics,General Immunology and Microbiology,General Biochemistry, Genetics and Molecular Biology,Modeling and Simulation,General Medicine

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