The Pattern of mRNA Expression Is Changed in Sinoatrial Node from Goto-Kakizaki Type 2 Diabetic Rat Heart

Author:

Howarth F. C.1ORCID,Qureshi M. A.1,Jayaprakash P.2,Parekh K.1,Oz M.2,Dobrzynski H.3,Adrian T. E.4

Affiliation:

1. Department of Physiology, College of Medicine & Health Sciences, UAE University, Al Ain, UAE

2. Department of Pharmacology, College of Medicine & Health Sciences, UAE University, Al Ain, UAE

3. Cardiovascular Sciences, University of Manchester, Manchester, UK

4. Department of Basic Medical Sciences, Mohammed Bin Rashid University of Medicine & Health Sciences, Dubai, UAE

Abstract

Background.In vivoexperiments in Goto-Kakizaki (GK) type 2 diabetic rats have demonstrated reductions in heart rate from a young age. The expression of genes encoding more than 70 proteins that are associated with the generation and conduction of electrical activity in the GK sinoatrial node (SAN) have been evaluated to further clarify the molecular basis of the low heart rate.Materials and Methods. Heart rate and expression of genes were evaluated with an extracellular electrode and real-time RT-PCR, respectively. Rats aged 12-13 months were employed in these experiments.Results. Isolated spontaneous heart rate was reduced in GK heart (161 ± 12 bpm) compared to controls (229 ± 11 bpm). There were many differences in expression of mRNA, and some of these differences were of particular interest. Compared to control SAN, expression of some genes were downregulated in GK-SAN: gap junction,Gja1(Cx43),Gja5(Cx40),Gjc1(Cx45), andGjd3(Cx31.9); cell membrane transport,Trpc1(TRPC1) and Trpc6 (TRPC6); hyperpolarization-activated cyclic nucleotide-gated channels,Hcn1(HCN1) andHcn4(HCN4); calcium channels,Cacna1d(Cav1.3),Cacna1g(Cav3.1),Cacna1h(Cav3.2),Cacna2d1(Cavα2δ1),Cacna2d3(Cavα2δ3), andCacng4(Cavγ4); and potassium channels,Kcna2(Kv1.2),Kcna4(Kv1.4),Kcna5(Kv1.5),Kcnb1 (Kv2.1),Kcnd3(Kv4.3),Kcnj2(Kir2.1),Kcnk1(TWIK1),Kcnk5(K2P5.1),Kcnk6(TWIK2), andKcnn2(SK2) whilst others were upregulated in GK-SAN:Ryr2(RYR2) andNppb(BNP).Conclusions. This study provides new insight into the changing expression of genes in the sinoatrial node of diabetic heart.

Funder

UAE University

Publisher

Hindawi Limited

Subject

Endocrinology,Endocrinology, Diabetes and Metabolism

Cited by 18 articles. 订阅此论文施引文献 订阅此论文施引文献,注册后可以免费订阅5篇论文的施引文献,订阅后可以查看论文全部施引文献

同舟云学术

1.学者识别学者识别

2.学术分析学术分析

3.人才评估人才评估

"同舟云学术"是以全球学者为主线,采集、加工和组织学术论文而形成的新型学术文献查询和分析系统,可以对全球学者进行文献检索和人才价值评估。用户可以通过关注某些学科领域的顶尖人物而持续追踪该领域的学科进展和研究前沿。经过近期的数据扩容,当前同舟云学术共收录了国内外主流学术期刊6万余种,收集的期刊论文及会议论文总量共计约1.5亿篇,并以每天添加12000余篇中外论文的速度递增。我们也可以为用户提供个性化、定制化的学者数据。欢迎来电咨询!咨询电话:010-8811{复制后删除}0370

www.globalauthorid.com

TOP

Copyright © 2019-2024 北京同舟云网络信息技术有限公司
京公网安备11010802033243号  京ICP备18003416号-3